Using molecular marker technology in studies on plant genetic diversity
Learning module - Volume 1
If required, download Acrobat Reader for viewing files.
Table of contents
- Title page (PDF 115 KB)
- Preface (PDF 9 KB)
- Foreword (PDF 11 KB)
- About this module (PDF 13 KB)
- Objectives (PDF 7 KB)
II. Protein-based technologies
III. DNA-based technologies
- DNA basics (PDF 394 KB)
- Restriction fragment length polymorphisms (RFLPs) (PDF 503 KB)
- PCR basics (PDF 326 KB)
- PCR with arbitrary primers (PDF 206 KB)
- Amplified fragment length polymorphisms (AFLPs) (PDF 279 KB)
- Sequence-tagged sites (Microsatellites etc) (PDF 308 KB)
- Latest strategies (PDF 324 KB)
IV. Complementary technologies (PDF 136 KB)
- Denaturing gradient gel electrophoresis (DGGE)
- Thermal gradient gel electrophoresis (TGGE)
- Single-stranded conformational polymorphism (SSCP)
- Heteroduplex analysis
- Denaturing high-performance liquid chromatography (DHPLC)
V. Final considerations (PDF 152 KB)
- Considerations when choosing a technique
- Practical applications
Download whole module (PDF in ZIP 2.66 MB)
