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Compiled by Luisa Cagelli
Istituto di Sperimentazione per la Pioppicoltura, 15033 Casale
Monferrato, Italy
1. Clone name/number
Name or number assigned when a clone is entered into the collection.
It serves as a unique identifier and is assigned whenever a clone is
entered into the collection. Letters should be used before the
name/number to identify genebank or national system:
ISP_N001 for the material maintained at ISP
(Casale Monferrato, Italy)
ISP_Brisighella for the material maintained at
ISP (Casale Monferrato, Italy)
INRA_71002 for the material maintained at INRA
(Orléans, France).
In this way codes used by the different
institutes involved in P. nigra genetic conservation (N001
for ISP Casale Monferrato, 71002 for INRA Orléans, etc.) can be
preserved with letters before the clone name/number to identify
institutes that maintain a clone.
It is important to use letters that identify
the institute and not just the country, because in some countries
there might be more than one institute involved in genetic
conservation.
2. Country where maintained
Country in which clone is maintained. International three-letter
standard abbreviations (codes) should be used, according to ISO
1988, N. 3166.
3. Institution where maintained
Name of institution in which clone is maintained. Abbreviations
adopted to identify institutions under "Clone name/number"
should be used.
4. Original clone name/number
Codes assigned from the institution of origin.
This is very important when the clone was obtained from another
institution. It could be useful to identify duplicates held in
different collections and should always accompany samples wherever
they are sent.
5. Collecting number
Original number assigned by collector of the sample.
This item could be useful to identify duplicates held in different
collections and should always accompany samples wherever they are
sent (this number may be different from the "Original clone
name/number").
6. Other name/number associated with the
clone
Any other identification numbers known for this clone.
6.1 Other name/number 1
6.2 Other name/number 2
6.3 Other name/number 3
6.4 Other name/number 4
6.5 Other name/number 5
7. Type of maintenance
0. None
1. Nursery stool-bed
2. Adult plantation
3. Pollen
4. Nursery stool-bed and adult plantation
5. Nursery stool-bed and pollen
6. Adult plantation and pollen
7. Nursery stool-bed, adult plantation and pollen
8. Notes
Any additional information should be specified here.
9. Collecting institution
Institution which originally collected the sample.
10. Country of origin
Country in which the sample was collected. International
three-letter standard abbreviations (codes) should be used,
according to ISO 1988, N. 3166.
11. Province/State
Name of the primary administrative subdivision of the country in
which the sample was collected.
12. Department/County
Name of the secondary administrative subdivision (within a
Province/State) of the country in which the sample was collected.
13. Location of collecting site
Name of the nearest town or village where the sample was originally
collected.
14. Collecting date of original sample
Year in which the sample was originally collected (YYYY).
15. Latitude of collecting site
Degrees and minutes followed by N (North)
(e.g. Casale Monferrato 45°07' North: 04507N)
16. Longitude of collecting site
Degrees and minutes followed by E (East) or W (West)
(e.g. Casale Monferrato 8°30' East: 00830E)
17. Elevation of collecting site
Altitude above sea level (m).
18. Sex
1. Female
2. Male
19. Female parent
When the female parent is known (clone obtained from artificial
crossing or from seed collected from known genotypes), the
"Original clone name/number" of the female parent should
be specified. Include the parents in the database even if they are
not currently present in stool-beds (the "Type of
maintenance" should then be "none").
20. Male parent
When the male parent is known (clone obtained from artificial
crossing), the "Original clone name/number" of the male
parent should be specified. Include the parents in the database even
if they are not currently present in stool-beds (the "Type of
maintenance" should then be "none").
General rules for compilation
Material to be included in the database
As poplar is propagated by means of cuttings it was decided to
include in the database only the clones that are propagated in
national collections. Three main types of clones should be included:
-
clones collected in the national territory
-
clones used as parents in controlled
crosses: it is important to include the parents of the clones
included in the database even if the parents are not currently
present in stool-beds (in this case specify "none" in
"Type of maintenance"), so as to have some information
about the origin of the clones obtained by open-pollination or
from artificial crossing
-
clones received from other countries if
the original name is known.
Type of sample originally collected
Although, as already specified, only clones must be included in the
database, two different types of samples might have been originally
collected: cuttings, if the clone derives from vegetative
propagation of a spontaneous tree, or seed if the clone derives from
seed collected from a spontaneous tree or from artificial crosses.
It is important to make this clear because a lot of the information
to be included in the passport database is related to the original
sample (collecting number, collecting institution, country of
origin, province/state, department/county, location of collecting
site, collecting date of original sample, latitude, longitude and
elevation of collecting site).
Exchange of data
It is important to follow some specific rules so that the
information from all the EUFORGEN members will be uniform.
Characters
Only the 26 CAPITAL LETTERS of the English alphabet should be used.
This is very important because some particular accented letters
existing in some alphabets are not available in computers.
File format
The information to be included in the database must be forwarded in
a file in ASCII format. The size of the fields is indicated in Table
1. If more characters are necessary for some particular fields (e.g.
the field "notes" reserved for additional information),
they can be enlarged but with specification in the note. If there is
more than one word in one field it is necessary to use an underscore
(_) between the words (e.g. CASALE_MONFERRATO).
Alternatively, the information to be included
in the database can be forwarded in a file in EXCEL format.
Missing data
Dash (-) should be inserted for every missing data: in some cases it
will probably be possible to complete the data later (sex, etc.).
Empty fields
Dash (-) should be inserted also in those fields which are
completely empty (such as, probably in many cases "Other name
associated to the clone") so as to have the same file structure
from every partner.
Geographic coordinates
The programme Arcinfo for the conversion of geographic coordinates
used by different countries can be used. Every participant shall
introduce latitude and longitude in the database according to the
projection system adopted in the respective country (specifing in a
note which system was adopted: e.g. in Italy the system used is
UTM). It will be possible to convert the coordinates later if
necessary.
Table 1. Size of fields (maximum
number of characters for each field).
No. |
Field name |
Max. number of characters |
| 1 |
Clone name/number |
20 |
| 2 |
Country where maintained |
3 |
| 3 |
Institution where maintained |
15 |
| 4 |
Original clone name/number |
20 |
| 5 |
Collecting number |
20 |
| 6 |
Other name/number associated
with the clone (1) |
20 |
| |
Other name/number associated
with the clone (2) |
20 |
| |
Other name/number associated
with the clone (3) |
20 |
| |
Other name/number associated
with the clone (4) |
20 |
| |
Other name/number associated
with the clone (5) |
20 |
| 7 |
Type of maintenance |
2 |
| 8 |
Notes |
100 |
| 9 |
Collecting institution |
20 |
| 10 |
Country of origin |
3 |
| 11 |
Province/state |
20 |
| 12 |
Department/county |
25 |
| 13 |
Location of collecting site |
25 |
| 14 |
Collecting date of original
sample |
4 |
| 15 |
Latitude of collecting site |
6 |
| 16 |
Longitude of collecting site |
6 |
| 17 |
Elevation of collecting site |
4 |
| 18 |
Sex |
2 |
| 19 |
Female parent |
20 |
| 20 |
Male parent |
20 |
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